Proliferation of somatic embryos. The embryos induced on
MS medium containing 4.14 μM picloram and 0.22 μM BA
were transferred to MS basal media containing various growth
regulator combinations (4.14 μM picloram+0.22 μM BA,
2.07 μM picloram+0.22 μM BA, 2.68 μM α-naphthalene
acetic acid [NAA]+0.89 μM BA, or 0.44–2.22 μM BA alone)
for further proliferation. Effect of different concentrations of
gelling agents (8, 10, 12, and 14 g/L agar or 2, 4, and 6 g/L
Phytagel™) were also studied