however , although none of the tested concentrations of alpha-tocopherol and ascorbic acid improved the subpopulation of acrosome intact and live cells and the subpopulation of sperm cells with plasma membrane stability compared with the control group and the lower concentration of ascorbic acid (0.45 g/L) ,
it is interesting to note that in our study , the same concentrations of these antioxidant increased the subpopulation of acrosome reacted and live sperm cells and decreased the subpopulation of YO-PRO-1 positive of nonviable sperm cells compared with control and the lower concentration of ascorbic acid.