Fed-batch SSF was conducted with initial solid loading of
approximately 7.78% (38.5 g wet Preparation of xylan-removed Sugarcane bagasse mixed with 100 mL of in
0.1 M citrate buffer (pH 4.8)) without prehydrolysis. 19.2 g of Preparation of xylan-removedwet substrate and the corresponding fresh enzymes were fed into
the mixture at 24 h, 60 h, and 96 h. The final solid loading was
14.6% (dry weight/v).