water between excisions. They were incubated in 50 mL of 0.4 M
mannitol solution and shaken at 1.67 Hz, at room temperature.
Initial electrolyte leakage of the solution was determined following
shaking for 3 h, using a conductivity meter. Final electrolyte
leakage was determined after autoclaving the material at 121 C for
1 h to release electrolytes. The percentage of electrolyte leakage