The fish were acclimatized in plastic aquaria
(50 30 38 cm) in a recirculation system (at 0.5 L/min) for at
least 2 weeks before the feeding trial. After the acclimatization, fish
with similar size (9.8 ± 0.04 g) were randomly chosen and
distributed into 12 tanks at a density of 24 fish per tank (436 fish/
m3). Fish were fed with basal diet (CK) or diets supplemented with
live (LY) or heat-inactivated yeast (HIY), with each diet randomly
assigned to four tanks.