2. Methods2.1. MaterialsChitinase (from Streptomyces griseus; EC. 3.2.1.14), shrimp shellchitin (pcode: 1001416772) sodium hydrogenphosphate, sodiumdihydrogenphosphate, sodium carbonate, sodium hydroxide, andethanol were obtained from Sigma-Aldrich. Potassium hexacyano-ferrate(III), methanol, and chloroform and HCl were purchasedfrom Merck.