At week 2, supplementing 3 and 5% FO in the diets elevated (P < 0.05) ALA contents by 17 and 29-fold over the control (18.8 mg/egg), respectively (Table 5). The same supplementations also resulted in (P < 0.05) approximately 3-fold increases in egg DHA (42.6 vs. 123 to 133 mg/egg), and increased egg EPA from undetectable in the control to 12.8 to 15.6 mg/egg. In comparison, supplementing 7.5 or 10% microalgae A produced no increases in ALA or EPA and little changes in DHA of eggs. Adding microalgae A to the 3 or 5% FO diets did not seem to further enrich any of the n − 3 fatty acids in eggs over the FO-supplemented alone diets. Effects of dietary FO and microalgae on the relative percentages of ALA, EPA, and DHA in the egg yolk fatty acids were similar to the above-described effects on the total egg contents of these n − 3 fatty acids, except for that microalgae additions produced increases (20 to 40%, P < 0.05) in the percentages of DHA (Supplemental Table S1).