There are two stages in the process separated by T shift at
critical time (tc): Proliferation (before T-shift) and Production
(after T-shift). In experiment 1, bioreactor pH was kept
constant and T was shifted after a critical cell density was
achieved. Simulated cell states were collected at each time
step and compared with cell cycle phase data collected from
experiments and analysed in the flow cytometer. In Fig. 4
comparison of VCD between experimental and simulation
results were illustrated for the three experiments. Model
showed good agreement with experimentally measured VCD
for all cases. For experiment 1 and 3, model prediction was
higher than the experimental findings before (tc) for a short
period of time. Better agreement for VCD was observed after
T shift for all cases.