The segregation ratios for all 6 transgenic lines for CHI and
Del/Ros1 were 3:1 at T1 generation. CHI (CHI-4, CHI-6, and
CHI-8) and Del/Ros1 (DR-4, DR-5, and DR-7) genes were recovered
from the transformation process. All transgenic tomatoes
were checked by genomic DNA amplification (Figure 2A). Once
the transgenic plants had the gene of interest, all were expressed for
both peel and flesh without exception (Figure 2B and C). There
was no distinguishable difference between lines regardless of the
flavonoid content in either the peel or the flesh.