3.5. Investigating Antibiotic Resistance
In order to determine antibiotic resistance, first 1% of
strains isolated from newly cultivated samples were added to warm sterile MRS agar with half concentration of
MacFarlane. Then, a fixed amount of the medium was divided into plates. After the cultivation medium was made
(i.e. became solid) antibiotic disks were placed at regular
intervals on plates and incubated in a CO2
incubator at 37
ºC. After 24 hours, each plate was retrieved and measured
using a metric rule for the area of inhabitation (11)