Two wavelengths selected for the method are 225 nm and
241 nm that are absorption maxima of ATL and ATV,
respectively in 50% v/v aqueous methanol. The stock
solutions of both the drugs were further diluted separately
with methanol to get a series of standard solution of
5-30 μg/mL concentration for ATL and 2-12 μg/mL
concentration for AVT. The absorbances were measured at
the selected wavelengths and absorptivities (A