The extractions were performed with a sample quantity of triturated hot Cayenne pepper of about 0.5 g, in 25mLof solvent, at a temperature of 125 ◦C (500W of power) for a period of 10 min and under magnetic stirring at 25%.
All the assays were conducted in triplicate.
Fig. 3 shows the relative chromatographic areas of the different capsaicinoids extracted with the five solvents tested.
It can be observed from the graph that methanol, ethanol and acetone could all equally be employed for the extraction of capsaicinoids giving similar results. It was decided to use ethanol since it is less toxic than the others.