GGB formation was first observed 2-3 weeks after incubation of the explants in GGB initiation medium. They exhibited a fresh green color with a glassy bead-like-shape (Figure 1A). Most of the GGBs were initiated from the basal region of the proximal explant sections or from adaxial surface of both sections. Pale brown rhizoids were seen growing abundantly around the GGBs (Figure 1B).