In the batch experiments, glucose and ammonium were depleted at the end of the exponential growth phase and a high cell lysis was observed. One fed-batch experiment was carried out without both glucose and ammonium at the end of the exponential growth phase and a high cell lysis was observed (experiment 1). To investigate if lysis was due to nutrients depletion (carbon or ammonium) two experiments were per-formed: experiment 2 and 3 with the addition at the end of the exponential phase of glucose and ammonium, respectively. The results, shown in Figure 4 and Table 1, indicate that glucose is totally consumed and ammonium accumulates in the media. Glucose is required for the sporulation stage, avoiding the autolytic tendencies
of the cells at the end of the exponential growth phase, in accordance with published data by [12] and [13].
In the batch experiments, glucose and ammonium were depleted at the end of the exponential growth phase and a high cell lysis was observed. One fed-batch experiment was carried out without both glucose and ammonium at the end of the exponential growth phase and a high cell lysis was observed (experiment 1). To investigate if lysis was due to nutrients depletion (carbon or ammonium) two experiments were per-formed: experiment 2 and 3 with the addition at the end of the exponential phase of glucose and ammonium, respectively. The results, shown in Figure 4 and Table 1, indicate that glucose is totally consumed and ammonium accumulates in the media. Glucose is required for the sporulation stage, avoiding the autolytic tendencies of the cells at the end of the exponential growth phase, in accordance with published data by [12] and [13].
การแปล กรุณารอสักครู่..
