The diluted sperm samples (10 μL) were diluted again with 200 μL of HEPES medium (20 mM Hepes, 197 mM NaCl, 2.5 mM KOH, and 10 mM glucose; pH 6.7 at room temperature, osmolality of 350 mOsm/kg).
The diluted sperm samples (10 μL) were diluted again with 200 μL of HEPES medium (20 mM Hepes, 197 mM NaCl, 2.5 mM KOH, and 10 mM glucose; pH 6.7 at room temperature, osmolality of 350 mOsm/kg).