A two-stage of dark and photo hydrogen production could be
used for conversion of crude glycerol to hydrogen. A
maximum cumulative hydrogen production of 64.24 mmol H2/
L was obtained from dark fermentation. In batch experiments,
the optimum condition for photo hydrogen production from
DFE was 5 times dilution of DFE without supplementation of
yeast extract þ NaHCO3 and 2mMglutamate. The overall yield
of 6.42 mmol H2/g COD consumed was achieved by the twostage
processes.