Finally, A11 strain showed 99% similarity with S. cerevisiae strain CBS 1907 (AJ508581) and 99% of identity with S. cerevisiae strain 810 GDB (EF554822). To confirm the position of each strain in phylogeny, a number of sequences were selected from the NCBI database for the construction of a phylogenetic tree using the MEGA4 program. As shown in Fig. 4 the isolates A2, A10 and A11 share the same clade cluster of the phylogenetic tree of D1/D2 26S rDNA sequences corroborating all they belong to the genus Saccharomyces.