Figure 5. Prevention of obesity by TNP-
470 in high fat diet-fed wt mice. Fiveweek-
old male C57Bl/6 mice were fed a
high-fat diet. Mice were systemically
treated with TNP-470 at a dose of 20
mg/kg or with vehicle alone for 12 weeks
(A to C). Food intake in the high-fat dietfed
mice with or without TNP-470 treatment
(A). Mouse body weight was measured
twice every week (B). Body length
was measured weekly (C). Autopsy
examination of adipose tissue distribution
was performed after 16-weektreatment.
Subcutaneous, perigonadal,
and omental fat depots and liver weights
were measured (D). Total fat/total body
weight (E) and lean mass/total body
weight (F) ratios were calculated. For
comparisons: *P0.05, **P0.01,
***P0.001.