4. Growth and lipase production in mini jar
fermenter
Production of lipase during growth of
Staphylococcus warneri PB233 was studied in
batch fermentation in a 2-liters mini jar fermenter
using SGC medium containing 1% olive oil and
3 M NaCl. A time course of lipase production from
this strain showed that the maximum lipase activity
(90.12 U/ml) was detected at 48 hr of incubation
at the stationary phase (Figure 3). The pattern of
lipase production from Staphylococcus warneri
PB233 was shown to be growth-associated (Lee
et al., 1999). In the last phase, growth was still
stationary, but enzyme activity was decreased. It
was possible that cells lost activity due to a high
concentration of enzyme (production inhibition)
(Kanlayakrit et al., 2001).