The E.coliand Entercoccus sp.strains were streaked for isolation on trypticase soy agar (TSA) and incubated for 24 h. Isolated coloneis were inoculataed into 10 ml of brain hear tinfution (BHI) broth and incubated for 24 h Each of thest cultules was then centrifuged at 5000rpm for 5 min(IEC multi, thermo scientific)