Therefore, the total treatment time was 5 min for continuous, 8 min for 10 s on/6 s off (300 s on (30 pulsed ‘on’ of 10 s)/180 s off (30 pulsed ‘off’ of 6 s), 10 min for 5 s on/5 s off (300 s on (60 pulsed ‘on’ of 5 s)/300 s off (60 pulsed ‘off’ of 5 s) and 25 min for 2 s on/8 s off (300 s on (150 pulsed ‘on’ of 2 s)/1500 s off (150 pulsed ‘off’ of 8 s)).