A small amount of industrial wastewater effluent produced from a pickled manufacturing plant (0.1 ml) was spread onto a sterilized surface plate of basal mineral salts BMS agar medium (3 g NaNO3, 1 g KH2PO4, 0.5 g MgSO4.7H2O, 0.5 g KCl, 1 g yeast extract and 20 g agar) containing 100 ml pickled wastewater as a sole of carbon source [2], these plates were incubated at 37% for 48 h.