grams of C. asiatica powder was soaked in 100 mL of 20% ethanol
and incubated at 37 C for 1 day. The ethanolic extract was filtered
and freeze-dried and the final CAE powder was stored in the dark
at 20 C until use.
2.3. HPLC analysis of C. asiatica extracts active compounds
C. asiatica (1 g) was extracted with 10 mL of 20 % ethanol at
37 C for 24 h. The extract was filtered with a 0.45-mm filter and
analyzed by HPLC. HPLC analyses were performed on a LC-2000
series apparatus (Jasco) with a PU-2089 plus pump and a MD-
2010 plus diode array detector, equipped with a LUNA C18 column
(250 4.6 mm inner diameter; 5-mm particle size; Phenomenex,
Torrance, CA, USA). The wavelength of diode array
detector was set at 206 nm. The analytical method was based on
previous study.