The fungal strains taken for the study were Candida albicans MTCC 1673,Candida tropicalis (Clinical Isolate), Aspergillus niger MTCC 21963, Microspourm gypseum MTCC 2829,Microspourm canis MTCC 2820. 0.1 ml of fungal strains culture
were spreaded over the Sabouraud Dextrose agar plates.