The reactor was equipped with a temperature sensor and a
water-heating system for temperature control. The experiment
was conducted at different temperatures, reducing from 35 C,
25 C to 15C. The reactor was operated at 35 C for 31 days, for
the following 33 days at 25 C, and for 37 days at 15 C. The water
permeation was kept at about 23.2 L/d in IAFMBR, which corresponded
to a hydraulic retention time (HRT) of 6 h. And 40 g GAC
(10 30 mesh) was added into the inner tube (AnMBR) of IAFMBR
to prevent membrane surface from the formation of biomass cake
and clogging. The membrane module was cleaned before a new
cycle each time (Gao et al., 2011b).