2.4.2. Stomatal traits
Portions of the epidermis were removed from the middle of the
leaf with a razor blade, stained with a mixture of 1:1 (v/v) 50%
aqueous ethanol and safranin (1% in water), and mounted in 1:1
(v/v) glycerol:water (Jensen, 1962). Slides were analyzed using an
Olympus DP71 microscope (Olympus Inc., Japan), and the single
stomatal pore area and stomatal pore area per unit were measured
using Motic Images Plus 2.0 (Wang et al., 2009). We analyzed 10
images per leaf, one leaf per plant and 5 plants per treatment. The
experiment was carried out 6 times.