In comparable experiments, vitamin D-deficient trout were
injected intraperitoneally with 25 ng (0.65 mCi) [26,27-3H]
1,25-dihydroxycholecalciferol dissolved in 100 ml propylene glycol.
The fish were killed 3 h later and tissues were collected for
determination of radioactivity. Again, the concentration of
excreted radioactivity in bile at 6.2 ng/ml was greater than in
blood plasma (0.17 ng/ml), kidneys (0.3 ng/g) and liver (0.9 ng/g),
and only about 20% of the radioactivity in bile appeared to be
unchanged 1,25(OH)2D3