3.2. Effect of T. harzianum BI on nematode egg hatching
Under in vitro condition, T. harzianum BI showed significantly effect on nematode egg hatching. Whereas maximum of egg
hatching percentage in control was at 3rd day, in treatments was at 6th day and was more or less 20% lower than their controls. So, T. harzianum BI not only reduced nematode egg production (as inoculum for second generation) but also caused egg weakness and mortality (Fig. 2).
3.3. PAL, POX and PPO enzymes activities in roots inoculated with T. harzianum BI and Meloidogyne javanica
Inoculation of tomato roots with T. harzianum BI significantly increased PAL, POX and PPO enzyme activities compared to control (inoculated with nematode). Maximum (peak) level of enzyme activities were at 6, 5 and 5 days after inoculation, respectively, and then slowly decreased (Figs. 3–5).