Cell
wall
polysaccharide
suspensions
(mainly
-glucan)
was
isolated
from
baker’s
yeasts
(
Saccharomyces
cerevisiae
)
and
used
for
the
preparation
of
films.
Glycerol
was
added
as
a
plasticizer.
Purity
and
composi-
tion
of
the
films
were
tested
by
elemental
analysis,
enzymatic
assay
of
-
and
-glucans,
monosaccharide
composition
analysis
(total
hydrolysis,
HPAEC)
and
vibration
spectroscopy
(FTIR,
FT
Raman).
Surface
properties
and
the
degree
and
type
of
crystallinity,
together
with
ageing
effects,
were
estimated
by
scan-
ning
electron
microscopy
(SEM),
atomic
force
microscopy
(AFM)
and
X-ray
diffraction
(XRD).
Mechanical
and
thermal
properties
were
characterized
by
tensile
tests
and
difference
scanning
calorimetry
(DSC),
respectively.
The
prepared
films
were
water
insoluble,
compact,
non-porous,
exhibit
no
pronounced
crystallinity
and
consist
of
granular-like
and
fibre
microstructures,
which
could
be
assigned
as
cell
wall
residues
and
released
polysaccharide
macromolecules.
Certain
structural
changes
in
the
film
surface
dur-
ing
one-year
shelf
storage
can
be
related
to
reorientation
and
decomposition
of
surface
macromolecules
due
to
reaction
with
the
ambient
atmosphere,
rather
than
to
crystallization
phenomena.
© 2011 Elsevier Ltd. All
Cell
wall
polysaccharide
suspensions
(mainly
?
-glucan)
was
isolated
from
baker’s
yeasts
(
Saccharomyces
cerevisiae
)
and
used
for
the
preparation
of
films.
Glycerol
was
added
as
a
plasticizer.
Purity
and
composi-
tion
of
the
films
were
tested
by
elemental
analysis,
enzymatic
assay
of
?
-
and
?
-glucans,
monosaccharide
composition
analysis
(total
hydrolysis,
HPAEC)
and
vibration
spectroscopy
(FTIR,
FT
Raman).
Surface
properties
and
the
degree
and
type
of
crystallinity,
together
with
ageing
effects,
were
estimated
by
scan-
ning
electron
microscopy
(SEM),
atomic
force
microscopy
(AFM)
and
X-ray
diffraction
(XRD).
Mechanical
and
thermal
properties
were
characterized
by
tensile
tests
and
difference
scanning
calorimetry
(DSC),
respectively.
The
prepared
films
were
water
insoluble,
compact,
non-porous,
exhibit
no
pronounced
crystallinity
and
consist
of
granular-like
and
fibre
microstructures,
which
could
be
assigned
as
cell
wall
residues
and
released
polysaccharide
macromolecules.
Certain
structural
changes
in
the
film
surface
dur-
ing
one-year
shelf
storage
can
be
related
to
reorientation
and
decomposition
of
surface
macromolecules
due
to
reaction
with
the
ambient
atmosphere,
rather
than
to
crystallization
phenomena.
© 2011 เอลส์ จำกัด ทั้งหมด
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