Hence, this method can be used for the clinical testing and pharmacokinetic studies. In order to study the matrix effect, Plasma and urine samples(both free of analyte) were diluted 10 and 5-fold, respectively, and spiked with the analyte at there concentration levels. The relative recoveries were calculated by comparing the obtained peak area of analyte in diluted samples with that of de-ionized water spiked at the same concentrations after the application of this method