(Difco, Detroit, MI, USA) is sufficient to relieve PCR inhibition (not shown) suggests that DPCR would provide a
rapid means to confirm the presence of STEC in enrichment
cultures. With optimized primer design and PCR condi- tions, DPCR could be used to detect other virulence genes
of E. coli O157:H7 and distinguish E. coli O157:H7 from
other STEC