Material and methods
Source of gametes
The rainbow trout and Black Sea trout gametes were
obtained from a private trout hatchery (Rize,Turkey).
Twelve female (3960 141g and 31 years old) and
eight male (1814 172 g and 21 years old) rainbow
trout, and15 female (904 76 and 21 years old) and
10male Black Sea trout (716 74 g and 21 years old)
were used. Gametes fromeach species were obtained
by conventional stripping methods, and these gametes
were bulked. Gametes were transferred to Rize
University Fisheries Faculty, Fish Breeding Research
Unit.These bulked gametes were used for intra-speci-
¢c and inter-speci¢c crossing.