A central composite rotatable design (CCRD) 2 with three central points and two star points for each independent variable was used in this work.
Coded and real values are shown in Table1.
The independent variables studied for the production of lipase were nitrogen concentration (0.32-0.88%w/w) and volume of liquid (60.9-89.1 ml)
Dependent variable was enzymatic activity (U/gdm)
Real values of these variables were selected based on preliminary studies.