The effect of moisture content of the feed material (84.6, 48.7, 17.5 and 0.8%) on the SC-CO2 extraction of carotenoids was tested at 70 °C, 55.1 MPa, and canola oil concentration of 5% (w/w). The particle size of feed material was maintained at 0.25–0.5 mm and the flow rate was fixed at 1 L/min. Extract fractions were collected every 30 min until 4 h. Extractions for each moisture level were carried out in duplicate and analyzed by HPLC.