In attached tiller assay and standard grain inoculation, lesion
development was observed within 15 days after inoculation. Development of sheath rot lesion was observed within 72
hours of inoculation with mycelial mat in detached leaf sheath assay and detached leaf assay. Seed inoculation, detached tiller assay and foliar inoculation with conidial suspension (105 conidia/ml) were recommended for large scale screening of
germplasm, breeding lines and phenotyping of mapping population.