It is known that contamination of AFM1 in milk is a result of exposure of AFB1 to dairy cattle through feedstuffs. Based on AFM1 contamination of milk, the calculated average concentration of AFB1 (according to Price et al. (1985)) in feeds consumed by the producing cows was 18.75 μg/kg, which is 3.8 times higher than the maximum allowed level in feeds for dairy cattle set by the European Directives (Directive 2002/32/EC (EC, 2002) and amending Directive 2003/100/EC (EC, 2003) to be 5 μg/kg. Additionally, levels of AFB1 in feeds consumed by the producing cows during February, April and May were 30, 16.25 and 10.63 μg/kg, respectively. However, this estimation is done considering a carry-over rate of 1.6% AFB1 as it is suggested (Price et al., 1985). However, taking into consideration reported (EFSA, 2004) carry-over rates that range from 2% (assumed average level) to 6% (reported level for high yielding cows) the extrapolated intake of AFB1 in feed is underestimated indicating that control measures need to be implemented in the initial food chain highlighting the monitoring of dairy products as well as feed provided to animals.