Prior to lab you should:
• Make sure you:
o know the relationship between proteins and amino acids
o know the “conserved region” of the amino acids
o know what an –R group is
o understand the basics of paper chromatography
How is it performed?
What causes hydrophilic molecules to migrate slower than hydrophobic
molecules?
what makes molecules hydrophobic/hydrophilic?
o are able to calculate an Rf factor