Purify lysolysase with a dual-water phase system (Na3C6H5O7/C3H8O) to explore the effect on the total protein distribution coefficient with environmental pH, reaction time, centrifugal temperature, centrifugal velocity, and pass lysosin through 15% (w/w) Sodium citrate and 16% (w/w) propylene alcohol are separated at 3000 rpm after 8 minutes of normal temperature reaction in a bihydrated phase system consisting of pH12, resulting in an optimal total protein distribution factor (K) of 7.279. ...
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