Extraction and concentration of anthocyanins pigment from purple carrots
Anthocyanins from purple carrots were extracted by using the method of Fuleki and Francis (1968) modified by Colin and Peter (1980).Twenty gm of purple carrots was mixed with 100 ml ethanol acidified with 0.01% citric acid and macerated in a warring blender at full speed for 5 min. The mixture was fil- tered by Whatman No. 1 filter paper through a Buchner funnel. The residue on the filter paper was washed rapidly with the extracting solvent until collecting of about 450 ml from extracted pigment carrot. The extract was concentrated in a rotary vacuum evaporator at >40 °C until the solvent evaporated.
Determination of total anthocyanin
A small liquate of the filtered extract was diluted with the extracting solvent to yield an optical density within the opti- mum range of the instrument. The diluted extract was stored in the dark for 2 h and absorbance was measured at 520 nm.