Main article: List of Resident Evil characters
Milla Jovovich as Alice
Michelle Rodriguez as Rain Ocampo
Eric Mabius as Matt Addison
James Purefoy as Spence Parks
Martin Crewes as Chad Kaplan
Colin Salmon as James "One" Shade
Ryan McCluskey as Mr. Grey
Oscar Pearce as Mr. Green
Indra Ové as Ms. Black
Anna Bolt as Dr. Green
Joseph May as Dr. Blue
Robert Tannion as Dr. Brown
Heike Makatsch as Dr. Lisa Addison
Stephen Billington as Mr. White
Fiona Glascott as Ms. Gold
Pasquale Aleardi as J.D. Salinas
Liz May Brice as Olga Danilova, The Medic
Torsten Jerabek as Vance Drew, Commando #1
Marc Logan-Black as Alfonso Warner, Commando #2
Michaela Dicker as Red Queen
Jason Isaacs as Dr. William Birkin / Narrator
Jeremy Bolt as Zombie
Barry Best as Zombie