in growth cabinet; however, the decline in seed oil content relative
toWTwas less pronounced for BnFUS3 mutant plants in the growth
cabinet experiment (Supplemental Figs. 6 and 7).
Analysis of FA composition by gas chromatography (GC)
revealed minute alterations in the relative levels of palmitic (16:0)
and stearic (18:0) acid content in seeds of some of the BnFUS3
TILLING mutant lines (Table 2). A consistent increase in linoleic acid
(18:2) was observed in all mutant lines (Table 2). A similar increase
was also found during the other experiment in the greenhouse and
with plants grown in the growth cabinet (Supplemental Tables 4
and 5).