This paper investigated if a reusable LED-illumination directly on all sides of disposable bags is possible. Besides the traditional cultivation of algae in sunlight illuminated vessels, the interest in cultivation using indoor-bioreactors grows. This development can be explained by the potential of producing high-value substances such as food additives, cosmetics and pharmaceuticals via algae. However, the requirements for the corresponding production are more complex with regard to sterility, process control and reproducibility. These requirements can be fulfilled in illuminated indoor photobioreactors. There are non-disposable commercial indoor photobioreactor systems available made of steel or glass to cultivate phototrophic organisms. For high-value products out of (mammalian) cell culture, disposable systems are more and more applied. This trend is also interesting for photobiotechnology. The illumination for disposable bags consists of reusable flexible LED-lines placed directly on all surfaces of the bag. This placement reduces light losses and illuminates the bag from all sides. Moreover, it is simple, space-saving and flexible. Cultivations of the organisms Chlorella vulgaris, applied as a well-known alga, and Euglena gracilis for the production of the substance paramylon demonstrated the suitability of such an illumination applied to a disposable Wave-system.