2.5. Two dim ensiona l thin layer chrom atography
1 or 2 mg of each one of the seven sampl es and one
standar d, hesp eridin, wer e dissol ved into 3 ml of methanol and spotted onto cellu lose plates (20 · 20 cm2, 0.1
mm thick). The plates were developed in the first direction, using EAW as solvent (80:20:40, ethyl acetate:acetic acid:water) and after drying, they were developed in
the second direction using 15% acetic acid (Mabry,
Markham, & Thomas, 1970). Spots were observed under UV light with and without the presence of ammonia
fumes.