Arachidonic acid (0.005 mmol/l), arachidic acid (0..25 mmol/l)
linoleic acid (0.25 mmol/l) and palmitic acid (1.35 mmol/l) had no
effect on the cryoresistance of spermatozoa. When spermatozoa were
frozen in the standard extender without fatty acids or in extenders
containing one of the described fatty acids the post-thaw motility
parameters and the post-thaw fertility were not significantly different.
The post-thaw motility rate amounted to 28.5±2.3% and the average
path velocity was 127.1±19.4 μm/s (data from control and treatments
pooled, n=12). From the motile spermatozoa 76.3±10.6% exhibited a
linear movement, 15.0±3.9% a non-linear movement and 8.7±9.5% a
circular movement. The post-thaw fertility did not differ between the
treatments and was 63.2±2.8% for the control (n=3) and 61.3±3.3%
for the cryopreservation treatments (data from treatments pooled,
n=9).