2.5. Statistical analysis
To confirm the effect of Tomarina1, the differences between the groups of same age were analyzed. To confirm the effect of ageing, differences between the same groups of 4-month or 8-month-old rats were evaluated. Comparisons between the groups were made using the Mann–Whitney U-test with Bonferroni correction. Since six pairwise comparisons were
performed, the level of significance was set at p < 0.0083. The data were analyzed using a statistical software package (SPSS 17.0J for Windows; SPSS Japan, Tokyo, Japan).