ABSTRACT: A monoclonal antibody-based immunodot test was compared to a polymerase chain
reaction (PCR) assay for managing white spot syndrome virus (WSSV) on shrimp farms at Kundapur
and Kumta situated in Udupi and Uttar Kannada Districts, respectively, of Karnataka on the west
coast of India. Of 12 grow-out farms in Kundapur, 6 (F1 to F6) yielded shrimp samples that were negative
for WSSV by both immunodot test and 1-step PCR from stocking to successful harvest. Samples
from the other 6 farms (F7 to F12) were positive for WSSV by both immunodot test and 1-step PCR at
various times post stocking, and their crops failed. In the 2 farms at Kumta (F13, F14), immunodot and
1-step PCR results were both negative, and harvests were successful. In contrast to 1-step PCR
results, farms F5, F6, F13, and F14 gave positive results for WSSV by 2-step PCR, and they were successfully
harvested at 105 d post stocking. Our results indicate that an inexpensive immunodot assay
can be used to replace the more expensive 1-step PCR assay for disease monitoring.
KEY WORDS: Penaeus monodon · WSSV · White spot syndrome virus · Monoclonal antibody ·
Immunodot assay · PCR · Polymerase chain reaction
ABSTRACT: A monoclonal antibody-based immunodot test was compared to a polymerase chainreaction (PCR) assay for managing white spot syndrome virus (WSSV) on shrimp farms at Kundapurand Kumta situated in Udupi and Uttar Kannada Districts, respectively, of Karnataka on the westcoast of India. Of 12 grow-out farms in Kundapur, 6 (F1 to F6) yielded shrimp samples that were negativefor WSSV by both immunodot test and 1-step PCR from stocking to successful harvest. Samplesfrom the other 6 farms (F7 to F12) were positive for WSSV by both immunodot test and 1-step PCR atvarious times post stocking, and their crops failed. In the 2 farms at Kumta (F13, F14), immunodot and1-step PCR results were both negative, and harvests were successful. In contrast to 1-step PCRresults, farms F5, F6, F13, and F14 gave positive results for WSSV by 2-step PCR, and they were successfullyharvested at 105 d post stocking. Our results indicate that an inexpensive immunodot assaycan be used to replace the more expensive 1-step PCR assay for disease monitoring.KEY WORDS: Penaeus monodon · WSSV · White spot syndrome virus · Monoclonal antibody ·Immunodot assay · PCR · Polymerase chain reaction
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