Here, we describe a faster and more successful anther
culture procedure for haploid production in Z. aethiopica. Our
preliminary research revealed certain factors that were crucial for
haploid plant regeneration from the cultured anthers. This paper
presents follow-up experiments that combined the crucial factors,
resulting in reproducible yields of haploid and DH plants. Proof is
provided that the plants originated from the microspores. Finally,
the performance of the DH plants is reported.