In vitro pasta digestion was carried out as described by Padalino
et al. (2013). Briefly, 5 g dried spaghetti were cut in 5.0 ± 1.0 cm
lengths and weighed. The spaghetti were boiled for the optimal
cooking time in a water bath containing 50 mL boiling water. The
spaghetti were tipped into a digestion vessel with 50 mL distiled
water and 5 mL maleate buffer (0.2 mol/L pH 6.0, containing
0.15 g/L CaCl2 and 0.1 g/L sodium azide) in a heating block at
37 C (GFL 1092, Germany) and allowed to equilibrate for 15 min.
Digestion was started by adding 0.1 mL