2.2.3. Total anthocyanins
Anthocyanins were extracted from 1 g of freeze-dried peel of
each fruit with 30 mL of 95% ethanol/1.5 M HCl (85:15, v:v)
(Moo-Huchin et al., 2014). The extract was transferred to a 50 mL
volumetric flask, completing the volume with 1.5 M ethanol–HCl
and stored for 12 h at 4 C. After filtration, the absorbance was
measured in a UV–Vis spectrophotometer PerkinElmer Lambda
11 at 535 nm. The total anthocyanin content was determined
applying the Lambert–Beer law, calculated as mg/100 g of DW,
through the formula: A535 dilution factor=E1%
1 cm;535 where A535 is
the absorbance in the diluted sample and E1%
1 cm;535 is the values factor
(98.2) of molar absorptivity for the acid–ethanol solvent and it
refers to the absorption of a mixture of cranberry anthocyanins in
acid–ethanol, measured in a 1 cm-cell at 535 nm, at a concentration
of 1% (w/v).