used consortia isolated from various natural sources. A more
promising approach is to use artificial microbial co-cultures formed
in an empirical way based on the analysis of their physiological
and metabolic properties. This approach enables developing bioreceptor
elements of biosensors with required consumer qualities
without time consuming experiments to isolate and identify natural
microorganisms.
The aim of this work was to search for a stable artificial microbial
co-culture with broad substrate specificity and to form an amperometric
BOD biosensor on its basis. The work used cells of the
yeasts Pichia angusta, Arxula adeninivorans, Debaryomyces hansenii,
Candida boidinii and of the bacteria Gluconobacter oxydans.